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Identification and functional analysis of a new phosphorylation site (Y398) in the SH3 domain of Abi-1
- Source :
-
FEBS Letters . Mar2011, Vol. 585 Issue 6, p834-840. 7p. - Publication Year :
- 2011
-
Abstract
- Abstract: Abi-1 is an adaptor protein for Abelson kinase (c-Abl), and Abi-1 promotes the Abl-mediated phosphorylation of Mammalian Enabled (Mena) by binding both c-Abl and Mena. Here, we identified a new phosphorylation site (Y398) in the SH3 domain of Abi-1, and disruption of Y398, combined with the previously identified phosphorylation site Y213, significantly weakens the binding of Abi-1 to c-Abl. The SH3 domain of Abi-1 and the proline-rich domain of c-Abl are involved in this interaction. Abi-1 phosphorylation at both sites stimulates the phosphorylation of Mena through the activation of c-Abl kinase. The phosphorylation of Abi-1 also plays a role in enhancing the adhesion of Bcr-Abl-transformed leukemic cells. Structured summary of protein interactions: Abi1 physically interacts with c-Abl by pull down (View Interaction 1, 2) c-Abl physically interacts with Abi1 by anti bait coimmunoprecipitation (View Interaction 1, 2) c-Abl physically interacts with Abi1 by pull down (View interaction) [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 00145793
- Volume :
- 585
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- FEBS Letters
- Publication Type :
- Academic Journal
- Accession number :
- 59327699
- Full Text :
- https://doi.org/10.1016/j.febslet.2011.02.012