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Simultaneous Quantification and Identification of Individual Chemicals in Metabolite Mixtures by Two-Dimensional Extrapolated Time-Zero 1H-13C HSQC (HSQC0).

Authors :
Kaifeng Hu
Westler, William M.
Markley, John L.
Source :
Journal of the American Chemical Society. 2/16/2011, Vol. 133 Issue 6, p1662-1665. 4p. 3 Graphs.
Publication Year :
2011

Abstract

Quantitative one-dimensional (1D) 1H NMR spectroscopy is a useful tool for determining metabolite concentrations because of the direct proportionality of signal intensity to the quantity of analyte. However, severe signal overlap in 1D 1H NMR spectra of complex metabolite mixtures hinders accurate quantification. Extension of 1D 1H to 2D 1H-13C HSQC leads to the dispersion of peaks along the 13C dimension and greatly alleviates peak overlapping. Although peaks are better resolved in 2D 1H-13C HSQC than in 1D 1H NMR spectra, the simple proportionality of cross peaks to the quantity of individual metabolites is lost by resonance-specific signal attenuation during the coherence transfer periods. As a result, peaks for individual metabolites usually are quantified by reference to calibration data collected from samples of known concentration. We show here that data from a series of HSQC spectra acquired with incremented repetition times (the time between the end of the first 1H excitation pulse to the beginning of data acquisition) can be extrapolated back to zero time to yield a time-zero 2D 1H-13C HSQC spectrum (HSQC0) in which signal intensities are proportional to concentrations of individual metabolites. Relative concentrations determined from cross peak intensities can be converted to absolute concentrations by reference to an internal standard of known concentration. Clustering of the HSQC0 cross peaks by their normalized intensities identifies those corresponding to metabolites present at a given concentration, and this information can assist in assigning these peaks to specific compounds. The concentration measurement for an individual metabolite can be improved by averaging the intensities of multiple, nonoverlapping cross peaks assigned to that metabolite. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00027863
Volume :
133
Issue :
6
Database :
Academic Search Index
Journal :
Journal of the American Chemical Society
Publication Type :
Academic Journal
Accession number :
58734174
Full Text :
https://doi.org/10.1021/ja1095304