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A targeted fimA DNA vaccine prevents alveolar bone loss in mice after intra-nasal administration.
- Source :
-
Journal of Clinical Periodontology . Apr2011, Vol. 38 Issue 4, p334-340. 7p. 1 Color Photograph, 2 Graphs. - Publication Year :
- 2011
-
Abstract
- Aim: To construct a dendritic cell (DC)-targeted DNA vaccine against FimA of Porphyromonas gingivalis and evaluate the immunogenicity and protection in mice. Materials and Methods: A targeted DNA plasmid pCTLA4-FimA, which encodes the signal peptide and extracellular regions of mouse cytotoxic T lymphocyte-associated antigen 4 (CTLA4), the hinge and Fc regions of human Igg1 and FimA of P. gingivalis, was constructed. Mice were immunized with pCTLA4-FimA, the non-targeted DNA plasmid pFimA, which contains only fimA gene, or pCI vector intra-nasally. Serum and saliva antibody responses were detected by enzyme-linked immunosorbent assay. The protection against P. gingivalis-induced periodontitis was evaluated by measuring alveolar bone loss in mice. Results: Mice immunized with pCTLA4-FimA showed elevated levels of specific serum IgG and salivary IgA antibody responses compared with mice immunized with pFimA (p<0.01). Both pFimA and pCTLA4-FimA immunized groups showed significantly lower alveolar bone loss, with the magnitude protection greater in the latter (p<0.01), compared with the pCI immunized group. Conclusions: The DC-targeted DNA construct pCTLA4-FimA enhanced both systemic and mucosal immunity following intra-nasal immunization. A DNA-based immunization strategy may be an effective way to attenuate periodontitis induced by P. gingivalis. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 03036979
- Volume :
- 38
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Journal of Clinical Periodontology
- Publication Type :
- Academic Journal
- Accession number :
- 58613158
- Full Text :
- https://doi.org/10.1111/j.1600-051X.2010.01700.x