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Cysteine-scanning mutagenesis of an eukaryotic pore-forming toxin from sea anemone.

Authors :
Anderluh, Gregor
Barlic, Ariana
Podlesek, Zdravko
Macek, Peter
Pungercar, Joze
Gubensek, Franc
Zecchini, Maria Lucia
Serra, Mauro Dalla
Menestrina, Gianfranco
Source :
European Journal of Biochemistry. Jul99 Part 1, Vol. 263 Issue 1, p128. 9p.
Publication Year :
1999

Abstract

Studies the topology of equinatoxin II, a eukaryotic pore-forming toxin from sea anemone Actinia equina by cysteine-scanning mutagenesis. Binding of avidin to lipid-bound biotinylated mutants; Production and characterization of cysteine mutants; Labeling of toxins in solution; Acrylodan as a probe of toxin-membrane interaction.

Details

Language :
English
ISSN :
00142956
Volume :
263
Issue :
1
Database :
Academic Search Index
Journal :
European Journal of Biochemistry
Publication Type :
Academic Journal
Accession number :
5839778
Full Text :
https://doi.org/10.1046/j.1432-1327.1999.00477.x