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Proteomic Profiles of Mesenchymal Stem Cells Induced by a Liver Differentiation Protocol.

Authors :
Leelawat, Kawin
Narong, Siriluck
Chaijan, Suthidarak
Khanit Sa-ngiamsuntorn
Disthabanchong, Sinee
Wongkajornsilp, Adisak
Hongeng, Suradej
Source :
International Journal of Molecular Sciences. Dec2010, Vol. 11 Issue 12, p4905-4915. 11p. 1 Black and White Photograph, 1 Chart, 4 Graphs.
Publication Year :
2010

Abstract

The replacement of disease hepatocytes and the stimulation of endogenous or exogenous regeneration by human mesenchymal stem cells (MSCs) are promising candidates for liver-directed cell therapy. In this study, we isolated MSCs from adult bone marrow by plastic adhesion and induced differentiation with a liver differentiation protocol. Western blot analyses were used to assess the expression of liver-specific markers. Next, MSC-specific proteins were analyzed with two-dimensional (2D) gel electrophoresis and peptide mass fingerprinting matrix-assisted laser desorption/ionization (MALDI)-time of flight (TOF)-mass spectrometry (MS). To confirm the results from the proteomic study, semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) analyses were performed. We demonstrated that MSCs treated with the liver differentiation protocol expressed significantly more albumin, CK19 and CK20, than did undifferentiated cells. In addition the results of proteomic study demonstrated increases expression of FEM1B, PSMC2 and disulfide-isomerase A3 in MSCs treated with the liver differentiation protocol. These results from proteomic profiling will not only provide insight into the global responses of MSCs to hepatocyte differentiation, but will also lead to in-depth studies on the mechanisms of proteomic changes in MSCs. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
16616596
Volume :
11
Issue :
12
Database :
Academic Search Index
Journal :
International Journal of Molecular Sciences
Publication Type :
Academic Journal
Accession number :
57847845
Full Text :
https://doi.org/10.3390/ijms11124905