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The role of microRNA-196a and microRNA-196b as ERG regulators in acute myeloid leukemia and acute T-lymphoblastic leukemia
- Source :
-
Leukemia Research . Feb2011, Vol. 35 Issue 2, p208-213. 6p. - Publication Year :
- 2011
-
Abstract
- Abstract: Overexpression of the ETS transcription factor ERG is an adverse prognostic factor in adult patients with acute myeloid leukemia (AML) and T-cell acute lymphoblastic leukemia (T-ALL). We investigated the regulation of ERG by microRNAs and explored their role in hematopoiesis and leukemia. Transfection of precursor molecules of miR-196a and miR-196b induced ERG downregulation and luciferase assays confirmed binding of miR-196a and miR-196b to the ERG 3′UTR. During in vitro differentiation of CD34+ cells, miR-196b expression decreased with time, indicating a role for miR-196b in early hematopoiesis. In AML, patients with NPM1-mutations had higher levels of miR-196a and miR-196b compared to NPM1-wildtype. In T-ALL patients, miR-196a and miR-196b expression was associated with an immature immunophenotype, and expression of CD34 and CD33. In conclusion, our results identify miR-196a and miR-196b as ERG regulators and implicate a potential role for these miRNAs in acute leukemia. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01452126
- Volume :
- 35
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Leukemia Research
- Publication Type :
- Academic Journal
- Accession number :
- 57516725
- Full Text :
- https://doi.org/10.1016/j.leukres.2010.05.007