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Depressed Biofilm Production in Bacillus amyloliquefaciens C06 Causes γ-Polyglutamic Acid (γ-PGA) Overproduction.
- Source :
-
Current Microbiology . Jan2011, Vol. 62 Issue 1, p235-241. 7p. 3 Color Photographs, 1 Diagram, 2 Charts, 2 Graphs. - Publication Year :
- 2011
-
Abstract
- Bacillus amyloliquefaciens C06, a potential agent in biological preservation of post-harvest fruit, was found to secrete extra-cellular γ-polyglutamic acid (γ-PGA) in liquid culture. In this work, M306, a transposon mutant of B. amyloliquefaciens C06, defective in forming structured colony and displaying enhanced ability of producing γ-PGA, was obtained. Inverse PCR and quantitative reverse transcription PCR (qRT-PCR) analysis demonstrated that the defective phenotype in M306 was associated with an ORF showing high similarity to RBAM_034550 from B. amyloliquefaciens FZB42. In this paper, the ORF was designated pbrA, standing for γ-PGA production and biofilm formation regulatory factor. qRT-PCR analysis also indicated that pbrA down-regulated mRNA expression of epsD and yqxM, the crucial genes involved in biofilm formation, but affected little on expression of ywtB, the gene directing γ-PGA synthesis. Evaluations in γ-PGA productivity of wild-type C06 and its mutants C06Δ epsA and C06Δ tasA, respectively, deficient in producing exopolysaccharides (EPS) and TasA, revealed that γ-PGA overproduction in M306 was probably due to the redistributed metabolic flux caused by defective production of EPS. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 03438651
- Volume :
- 62
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Current Microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 56525144
- Full Text :
- https://doi.org/10.1007/s00284-010-9696-0