The effects of vortex breakdown bubbles on the mixing environment inside a base driven bioreactor

Abstract: The bubble-type vortex breakdown inside a cylinder with flow driven by rotation of the base, has applications in mixing. We investigate this phenomena and its effect on the environment inside an open cylinder, with potential application as a tissue-engineering bioreactor, with tissue-scaffolds of two different geometries immersed in the fluid. Addition of scaffolds induces a blockage effect, hindering the flow in the central vortex core returning to the rotating base. This promotes early onset of vortex breakdown and alters the final shape of vortex breakdown bubbles. Placement of the scaffolds centrally on the cylinder axis yields almost identical levels and distributions of shear stress between the upper and lower surfaces of scaffolds. A change from a disk shaped to an ellipsoidal scaffold, of the same size, reduces the intensity of the maximum shear stresses at the scaffold surface by up to 50%. There is a range of Reynolds numbers where increasing Reynolds number, and hence possibly increasing mixing efficiency, leads to a decrease in the maximum levels of fluid forces at the scaffold surfaces. This is an important conclusion for scaffold based tissue engineering where improved mixing is sought, but often sacrificed in favor of minimizing fluid forces. [Copyright &y& Elsevier]