Back to Search
Start Over
SELDI-TOF serum proteomics and colorectal cancer: A current overview.
- Source :
-
Archives of Physiology & Biochemistry . Oct2010, Vol. 116 Issue 4/5, p188-196. 9p. - Publication Year :
- 2010
-
Abstract
- Surface enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) is a widely used technology platform for diagnostic biomarker discovery in tissue, plasma and serum. High-throughput and simplicity of experimental procedures have allowed this technology to become an important research tool for biomarker discovery during the last years. This review provides an overview of SELDI-TOF functionality, its advantages and drawbacks and gives a current literature overview of colorectal cancer based serum biomarker detection. Further improvements in instrumentation sensitivity and labelling chemistries will enable detection of novel, tissue-leakage biomarkers in serum. However, major emphasis should be given on subsequent identification of differentially observed protein peaks detected by SELDI-TOF. Clinical validation in large patient cohorts will then allow transferring novel biomarkers into clinical use. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 13813455
- Volume :
- 116
- Issue :
- 4/5
- Database :
- Academic Search Index
- Journal :
- Archives of Physiology & Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 55287138
- Full Text :
- https://doi.org/10.3109/13813455.2010.495130