C-Terminal Truncation and Histidine-Tagging of Cytochrome c Oxidase Subunit II Reveals the Native...

Authors :: Hiser, Carrie
Mills, Denise A.
Schall, Michael
Ferguson-Miller, Shelagh
Source :: Biochemistry. 02/13/2001, Vol. 40 Issue 6, p1606. 10p. 2 Black and White Photographs, 2 Diagrams, 2 Charts, 6 Graphs.
Publication Year :: 2001
Abstract: Characterizes C-terminal truncation and histidine-tagging of cytochrome c oxidase subunit II of the photosynthetic bacteria Rhodobacter sphaeroides. Removal of excess lipids in empty vesicles; Purification of oxidase-containing vesicles by metal affinity column; Factors involved in the modification of chromatographic behavior of cytochrome c.

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