Back to Search
Start Over
DNA in situ hybridization on polytene chromosomes ofSimulium sanctipauli at loci relevant to insecticide resistance.
- Source :
-
Medical & Veterinary Entomology . Jun2000, Vol. 14 Issue 2, p217. 6p. - Publication Year :
- 2000
-
Abstract
- A DNA technique for in situ hybridization developed by Kumar & Collins (1994) for use on polytene chromosomes of adult Anopheles mosquitoes (Diptera: Culicidae) was modified for use with Simulium larval salivary gland chromosomes (Diptera: Simuliidae). Cloned fragments of several Simulium genes (coding for aspartate amino transferase, cytochrome P450 and DNA polymerase) were successfully mapped physically by assigning specific band locations in Simulim sanctipauli V. & D. This represents the first attempt at locating genes beyond the resolution of linkage to inversions in any blackfly species. [ABSTRACT FROM AUTHOR]
- Subjects :
- *SIMULIUM
*IN situ hybridization
*GIANT chromosomes
Subjects
Details
- Language :
- English
- ISSN :
- 0269283X
- Volume :
- 14
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Medical & Veterinary Entomology
- Publication Type :
- Academic Journal
- Accession number :
- 5519284
- Full Text :
- https://doi.org/10.1046/j.1365-2915.2000.00230.x