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Deletion of Tis7 Protects Mice from High-Fat Diet-Induced Weight Gain and Blunts the Intestinal Adaptive Response Postresection.
- Source :
-
Journal of Nutrition . Nov2010, Vol. 140 Issue 11, p1907-1914. 8p. - Publication Year :
- 2010
-
Abstract
- After loss of intestinal surface area, the remaining bowel undergoes a morphometric and functional adaptive response. Enterocytic expression of the transcriptional coregulator tetradecanoyl phorbol acetate induced sequence 7 (Tis7) is markedly increased in a murine model of intestinal adaptation. Mice overexpressing Tis7 in intestine have greater triglyceride absorption and weight gain when fed a high-fat diet (42% energy) than their wild-type )WT) littermates fed the same diet. These and other data suggest that Tis7 has a unique role in nutrient absorptive and metabolic adaptation. Herein, male Tis-/- and WT mice were fed a high-fat diet (42% energy) for 8 wk. Weight was monitored and metabolic analyses and hepatic and intestinal lipid concentrations were compared after 8 wk. Intestinal lipid absorption and metabolism studies and intestinal resection surgeries were performed in separate groups of Tis-/- and WT mice. At 8 wk, weight gain was less and jejunal mucosal and hepatic triglyceride and cholesterol concentrations were lower in Tis-/- mice than in the WT controls. Following corn oil gavage, serum cholesterol, triglyceride, and FFA concentrations were lower in the Tis-/- mice than in the WT mice. Incorporation of oral ³[H] triolein into intestinal mucosal cholesterol ester and FFA was less in Tis-/- compared with \NT mice. Following resection, crypt cell proliferation rates and villus heights were lower in Tis-/- than in WT mice, indicating a blunted adaptive response. Our results suggest a novel physiologic function for Tis-/- in the gut as a global regulator of lipid absorption and metabolism and epithelial cell proliferation. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00223166
- Volume :
- 140
- Issue :
- 11
- Database :
- Academic Search Index
- Journal :
- Journal of Nutrition
- Publication Type :
- Academic Journal
- Accession number :
- 54902528
- Full Text :
- https://doi.org/10.3945/jn.110.127084