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Nicking endonuclease and target recycles signal amplification assisted quantum dots for fluorescence detection of DNA

Nicking endonuclease and target recycles signal amplification assisted quantum dots for fluorescence detection of DNA

Authors :
Niu, Shuyan
Li, Quanyi
Qu, Lijing
Wang, Wei
Source :
Analytica Chimica Acta. Nov2010, Vol. 680 Issue 1/2, p54-58. 5p.
Publication Year :
2010

Abstract

Abstract: An ultrasensitive fluorescence detection method for DNA based on nicking endonuclease (NEase) and target recycles assisted with CdTe quantum dots (QDs) is reported. In the detection system, when the target DNA is present, it hybridizes with a linker strand to from a duplex, in which the NEase recognizes specific nucleotide sequences and cleaves the linker strand. After nicking, the fragments of the linker strand spontaneously dissociate from the target DNA and another linker strand hybridizes to the target to trigger another strand-scission cycle. On the other hand, when the target was absent, no duplex is formed and no fragment of linker strand is produced. Then CdTe QDs and magnetic beads (MBs), which were all modified with DNA sequences complementary to that of the linker strands are added to the solution to detect the presence of a target DNA. The signal was generated through the difference in Förster resonance energy transfer (FRET) between the MB and CdTe QDs. This method indicates that one target DNA leads to cleavage of hundreds of linker DNA, increasing detection sensitivity by nearly three orders of magnitude. This method should be applicable whenever there is a requirement to detect a specific DNA sequence and can also be used for multicomponent detection. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00032670
Volume :
680
Issue :
1/2
Database :
Academic Search Index
Journal :
Analytica Chimica Acta
Publication Type :
Academic Journal
Accession number :
54607698
Full Text :
https://doi.org/10.1016/j.aca.2010.09.025