Formación de callos y establecimiento de suspensiones celulares embriogénicas de caña de azúcar a partir de segmentos de hojas de plantas in vitro.

The somatic embryogenesis propagation technique and tool for the genetic improvement in sugar cane (Saccharum spp. hybrid) has presented multiple inconveniences that the regeneration protocols described in the literature have not been able to solve. The present research was carried out with the objective of developing a new methodology for the establishment of cell suspensions of sugar cane, var. C 87-51, through somatic embryogenesis based on segments of the sheath of the leaves of in vitro plants, placed directly in a liquid culture medium. The results demonstrated that it is possible to form callus with embryogenic structures based on segment two of the sheath of the leaves which reduces the time of culture in vitro and guarantees the physiologic and phytosanitary quality of the plants that are regenerated. Homogeneous lines of cellular suspensions formed by embryogenic aggregates were established based on callus with embryogenic structures. [ABSTRACT FROM AUTHOR]