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MHC II on transfused murine blood is not required for alloimmunization against MHC I.
- Source :
-
Vox Sanguinis . Nov2010, Vol. 99 Issue 4, p369-374. 6p. 1 Diagram, 3 Graphs. - Publication Year :
- 2010
-
Abstract
- Background and Objectives Transfusion of allogeneic platelet products can result in antibodies against donor major histocompatibility complex (MHC) I antigens, leading to a refractory state to subsequent platelet transfusions. However, there is disagreement in the field regarding the molecular mechanisms of humoral alloimmunization. One hypothesis states that donor MHC II is a requirement for alloimmunization. However, other studies have suggested that donor MHC I is alone sufficient and MHC II is not required. Materials and Methods We utilized a mouse model of anti-MHC I alloimmunization to transfused blood, which employed donors with a complete deletion of all MHC II genes. BALB/c (H-2d) recipients were transfused with blood from either C57BL/6 (H-2b) or MHC II null donors on a C57BL/6 background. Anti-MHC I alloimmunization was monitored by indirect immunofluorescence. Results Recipients of either wild type or MHC II null blood produced equivalent humoral responses against donor MHC I antigens. However, there was variation in the relative amounts of IgG subclasses. Conclusion These data reject the hypothesis that donor MHC II expression is required for alloimmunization to MHC I antigens. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00429007
- Volume :
- 99
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Vox Sanguinis
- Publication Type :
- Academic Journal
- Accession number :
- 54418068
- Full Text :
- https://doi.org/10.1111/j.1423-0410.2010.01351.x