Sistema de diagnóstico computadorizado para la clasificación del estrés oxidativo en un modelo de sepsis peritoneal en ratas.

Great numbers of experimental studies have argued about the relevant role of the oxidative stress to the organ failure occurring in sepsis and endotoxic shock. However , there are no reports about the classification of oxidative stress grade of the affected organs. This elucidation is very important for the knowledge of involved mechanisms in the sequence of multi-organ failure. The present study compares results from conventional evaluation of redox biomarkers with those obtained from utilization of a computer system for diagnosis to classify the grade of oxidative stress present in liver and lung. Liver and lung were the organs studied from rats with peritoneal sepsis induced by faecal material. Organs were extracted 12 h after induction of sepsis. Conventional analysis showed that only thiobarbituric acid reactive substances and catalase were significantly increased in the hepatic tissue of septic animals. No significant changes in conjugated dienes, glutathione peroxidase and superoxide dismutase were found. In the lung of these animals were found no significant difference in all biomarkers. Values from such biomarkers were introduced in the computer system. Evaluation by the system showed oxidative stress with significant differences from statistical and biological point of view in the liver and lung of septic animals. A classification of grade 3 (severe oxidative stress) in the liver and grade 2 (moderated oxidative stress) in the lung was obtained from the system. These results suggest that hepatic tissue is before affected than the lung in the sequence of organic failure propagation, and demonstrate the utility in using the computer system for oxidative stress evaluation in vivo. [ABSTRACT FROM AUTHOR]