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Dumbbell-like Au-Fe3O4 nanoparticles as label for the preparation of electrochemical immunosensors
- Source :
-
Biosensors & Bioelectronics . Oct2010, Vol. 26 Issue 2, p627-631. 5p. - Publication Year :
- 2010
-
Abstract
- Abstract: In this work, we developed a novel kind of label based on dumbbell-like Au-Fe3O4 nanoparticles (NPs) for the preparation of electrochemical immunosensor for the detection of cancer biomarker prostate specific antigen (PSA). The signal amplification strategy, using the synergetic effect present in Au-Fe3O4 to increase the reduction ability of the NPs toward H2O2, improves the sensitivity and detection limit of the immunosensor. With primary anti-PSA antibody (Ab1) immobilized onto graphene surface and secondary anti-PSA antibody (Ab2) adsorbed onto the Au of the Au-Fe3O4 NPs, the immunosensor prepared through a sandwich structure displays a wide linear range (0.01–10ng/mL), low detection limit (5pg/mL), good reproducibility and stability. These labels for immunosensors may provide many potential applications for the detection of different biomolecules. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09565663
- Volume :
- 26
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Biosensors & Bioelectronics
- Publication Type :
- Academic Journal
- Accession number :
- 53969444
- Full Text :
- https://doi.org/10.1016/j.bios.2010.07.012