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Construction of a bioluminescence reporter plasmid for Francisella tularensis
- Source :
-
Plasmid . Nov2010, Vol. 64 Issue 3, p156-161. 6p. - Publication Year :
- 2010
-
Abstract
- Abstract: A Francisella tularensis shuttle vector that constitutively expresses the Photorhabdus luminescens lux operon in type A and type B strains of F. tularensis was constructed. The bioluminescence reporter plasmid was introduced into the live vaccine strain of F. tularensis and used to follow F. tularensis growth in a murine intranasal challenge model in real-time by bioluminescence imaging. The results show that the new bioluminescence reporter plasmid represents a useful tool for tularemia research that is suitable for following F. tularensis growth in both in vitro and in vivo model systems. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 0147619X
- Volume :
- 64
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Plasmid
- Publication Type :
- Academic Journal
- Accession number :
- 53795744
- Full Text :
- https://doi.org/10.1016/j.plasmid.2010.07.001