Competition between histamine-like and poly-imidazole coordination sites for Cu2+and Zn2+ions in zebra-fish peptide of prion-like proteinElectronic supplementary information (ESI) available: 1H and 13C NMR chemical shift of zf74-86 1.0 mM, pH 7.5, T298 K; UV-Vis spectra of Cu2+–zf74-86 complexes at different pH. Metal to ligand ratio = 1 : 1.1; intensity reduction of 1H–1H TOCSY cross-peaks of zf74-86 1.0 mM solutions induced by 0.3 equivalents of Cu2+, pH 3.3, T298 K; ESI-MS spectra of zf74-86. See DOI: 10.1039/c0dt00137f

The fragment of the zebrafish prion-like protein (PrP-rel-2), encompassing residues 74-86 and unprotected at N-terminus (zf74-86) represents a good model to understand Cu2+and Zn2+binding to ligands containing multi-potential metal donor sites. Zf(74-86) contains four His and His-1 N-terminal amine groups which constitute both copper and zinc anchoring sites. The presence of His at the first position additionally provides the histamine-like binding mode which could compete with the multi-His binding mode. In this study the speciation profiles of the Cu2+and Zn2+complexes with zf74-86 have been obtained. The main species, dominating at physiological pH, have been fully characterized by using different spectroscopic techniques. The detected NMR chemical shift variations and line broadening enhancements, caused by Zn2+and Cu2+respectively, allowed to determine the metal binding sites. Both metal ions showed common binding donor atoms, being 2 or 3 His imidazoles and the N-terminal group involved in Cu2+and Zn2+binding. [ABSTRACT FROM AUTHOR]