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Identification and characterization of a serine protease inhibitor of Clonorchis sinensis

Authors :
Kang, Jung-Mi
Sohn, Woon-Mok
Ju, Jung-Won
Kim, Tong-Soo
Na, Byoung-Kuk
Source :
Acta Tropica. Nov2010, Vol. 116 Issue 2, p134-140. 7p.
Publication Year :
2010

Abstract

Abstract: A gene encoding a serine protease inhibitor of Clonorchis sinensis (CsSERPIN) was identified and characterized. CsSERPIN contained an open reading frame of 1158bp that encoded 385 amino acid residues. Sequence analysis of the primary structure of CsSERPIN revealed that it had essential structural motifs including a reactive central loop (RCL), which well conserved in the serine protease inhibitor (serpin) superfamily. CsSERPIN was classified as a member of the ovalbumin-type serpin family on the basis of phylogenetic analysis and the absence of a classical N-terminal signal peptide. Recombinant CsSERPIN showed an inhibitory effect on chymotrypsin in a dose-dependent manner, but did not effectively inhibit trypsin, thrombin, elastases or cathepsin G. Optimal pH values of CsSERPIN were between 7.0 and 9.0, as evidenced by the rapid loss of inhibitory activity under acidic conditions. CsSERPIN was expressed at various developmental stages of the parasite, from eggs to adult worms, but its expression level was higher in eggs and adult worms than in metacercariae and juvenile worms. CsSERPIN was identified in the soluble extract of the parasite, but not in the excretory and secretory products (ESP) or insoluble extract of the parasite. Immunolocalization analysis of CsSERPIN showed that it mainly localized to the eggs and vitelline glands of the adult worm. These results suggest that intracellular CsSERPIN may be possibly involved in maintaining the physiology of eggs as well as in egg production of C. sinensis by regulating endogenous serine proteases. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
0001706X
Volume :
116
Issue :
2
Database :
Academic Search Index
Journal :
Acta Tropica
Publication Type :
Academic Journal
Accession number :
53708733
Full Text :
https://doi.org/10.1016/j.actatropica.2010.06.007