Preparation in supercritical CO2 of porous poly(methyl methacrylate)–poly(l-lactic acid) (PMMA–PLA) scaffolds incorporating ibuprofen

Abstract: Partially biodegradable porous scaffolds incorporating bioactive molecules prepared by clean techniques posses an enormous interest in tissue engineering applications. Poly(methyl methacrylate)–poly(l-lactic acid) (PMMA–PLA) blends were submitted to CO2 supercritical conditions (P =160–260bar, T =60°C) after certain time and then rapidly depressurized to obtain porous structures that have been related with the supercritical parameters and to the polymer blend composition. In some cases ibuprofen was also incorporated to the formulations previously to the CO2 treatment and studied the appropriate conditions for avoiding its extraction in SCCO2. Scaffolds purity, thermal transitions, swelling and degradation behaviour, and the ibuprofen release were also studied to determine the appropriate scaffolds with a desired porosity for cell seeding. Cell culture was performed on the selected porous scaffolds using human fibroblast examined by scanning electron microscopy (SEM). [Copyright &y& Elsevier]