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Silencing of the hypoxia inducible factor 1 –HIF-1- obliterates the effects of hypoxia on glucose and lactate concentrations in a tissue-specific manner in the shrimp Litopenaeus vannamei
- Source :
-
Journal of Experimental Marine Biology & Ecology . Sep2010, Vol. 393 Issue 1/2, p51-58. 8p. - Publication Year :
- 2010
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Abstract
- Abstract: Many molecular and physiological responses to hypoxia are controlled by the transcription factor called hypoxia inducible factor 1 (HIF-1). Metabolic responses are particularly important for marine crustaceans that suffer hypoxia in their aquatic environment, including shrimp. HIF-1 is a heterodimer recently described in shrimp and composed by α and β subunits. In the present study, we investigated the effect of silencing HIF-1 using dsRNA in the response to hypoxia in the shrimp Litopenaeus vannamei in vivo. Intramuscular injection of dsRNA for α and β separately, decreased the concentration of the corresponding transcripts in gills from shrimp maintained in normoxia, while no effect was detected in muscle. In contrasts, during hypoxia, silencing of HIF-1α or HIF-1β, did affect the corresponding transcripts. Moreover, silencing of the α and β subunits of HIF-1 affected the concentrations of glucose and lactate in hemolymph and gills from shrimp exposed to hypoxic conditions, giving insights of the action of HIF-1 in these responses. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00220981
- Volume :
- 393
- Issue :
- 1/2
- Database :
- Academic Search Index
- Journal :
- Journal of Experimental Marine Biology & Ecology
- Publication Type :
- Academic Journal
- Accession number :
- 53408476
- Full Text :
- https://doi.org/10.1016/j.jembe.2010.06.031