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The analgesic and anti-inflammatory effect of WIN-34B, a new herbal formula for osteoarthritis composed of Lonicera japonica Thunb and Anemarrhena asphodeloides BUNGE in vivo
- Source :
-
Journal of Ethnopharmacology . Sep2010, Vol. 131 Issue 2, p485-496. 12p. - Publication Year :
- 2010
-
Abstract
- Lonicera japonica Thunb and Anemarrhena asphodeloides BUNGE have been used for the treatment of a variety of inflammatory diseases, cold and infective diseases in many countries, including Korea and China. Aim of the study: : This study aimed to assess the anti-nociceptive and anti-inflammatory activities of n-butanol fraction (WIN-34B) prepared from dried flowers of Lonicera japonica and dried roots of Anemarrhena asphodeloides as potential novel treatment of osteoarthritis. Materials and methods: : Anti-nociceptive activities of WIN-34B (100, 200 and 400mg/kg, p.o.) were measured using acetic acid-induced writhing response, formalin-induced paw licking, hot plate, radiant heat tail-flick, carrageenan-induced paw pressure, and Hargreaves tests, respectively. Anti-inflammatory activities of WIN-34B (100, 200 and 400mg/kg, p.o.) were assessed using acetic acid-induced vascular permeability, carrageenan-induced paw edema, and croton oil-induced ear edema. Anti-osteoarthritis effect of WIN-34B was analyzed using monosodium iodoacetate (MIA)-induced osteoarthritis animal model. Results: : WIN-34B exhibited better anti-inflammatory activity than that of celecoxib in carrageenan at the dose of 200mg/kg and croton oil-induced paw edema and ear edema at the doses of 200 and 400mg/kg. WIN-34B exhibited significant anti-inflammatory effects on vascular permeability. WIN-34B also exhibited significant anti-nociceptive activities in the late phase of formalin-induced paw licking and writhing response model in mice. In radiant heat tail-flick and carrageenan-induced paw pressure tests, WIN-34B at the dose of 400mg/kg and at the doses of 200 and 400mg/kg presented similar activities to indomethacin and celecoxib. Compared to indomethacin WIN-34B at 400mg/kg showed similar or better anti-nociceptive activities after 1 and 2h of theraphy in the hot plate test and better anti-nociceptive activity than that of celecoxib in Hargreves test. In the MIA-induced osteoarthritis animal models, WIN-34B at 400mg/kg exhibited similar or better anti-nociceptive property than that of celecoxib throughout the pain measurement periods. Conclusion: : When compared to celecoxib, WIN-34B exhibited similar or better anti-nociceptive and anti-inflammatory activities in osteoarthritic animal models, which may become a potential novel treatment for osteoarthritis. [Copyright &y& Elsevier]
- Subjects :
- *ETHNOPHARMACOLOGY
*BODY fluid disorders
*NONSTEROIDAL anti-inflammatory agents
*DRIED flowers
*MEDICINAL plants
*OSTEOARTHRITIS treatment
*THERAPEUTICS
*OSTEOARTHRITIS
*PAIN
*PREVENTIVE medicine
*ALTERNATIVE medicine
*ANALGESICS
*ANALYSIS of variance
*ANIMAL experimentation
*ANTI-inflammatory agents
*BIOPHYSICS
*COMPUTER software
*DRUG toxicity
*FLOWERS
*LIQUID chromatography
*RESEARCH methodology
*MICE
*RATS
*RESEARCH funding
*PLANT roots
*SCIENTIFIC method
*SCIENTIFIC apparatus & instruments
*STATISTICS
*PHYTOCHEMICALS
*PLANT extracts
*DATA analysis
*PHARMACODYNAMICS
*PREVENTION
Subjects
Details
- Language :
- English
- ISSN :
- 03788741
- Volume :
- 131
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Journal of Ethnopharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 53407068
- Full Text :
- https://doi.org/10.1016/j.jep.2010.07.025