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Genetic and serological analyses of elongation factor EF-Tu of paulownia witches’-broom phytoplasma (16SrI-D).
- Source :
-
Plant Pathology . Oct2010, Vol. 59 Issue 5, p972-981. 10p. 1 Diagram, 2 Charts. - Publication Year :
- 2010
-
Abstract
- This study determined the tuf gene sequence of the phytoplasma specific to paulownia witches’-broom from Nanyang, China (hereby designated PaWB-Ny). The PaWB-Ny tuf gene was 1185 nucleotides in length and confirmed that the phytoplasma belongs to subgroup 16SrI-D of aster yellows. Three characteristic GTP-binding protein motifs were identified based on the peptide deduced from the tuf gene sequence. Results suggested that the elongation factor EF-Tu was localized in the cytoplasm and lacked hydrophobic transmembrane domains. Antibodies against PaWB-Ny EF-Tu were prepared by rabbit immunization with glutathione-S-transferase (GST)-tagged EF-Tu fusion protein expressed in Escherichia coli. EF-Tu exhibited a molecular weight of ∼43 kDa and was detected in PaWB-infected paulownia plants by western blot analysis. Indirect enzyme-linked immunosorbent assays (ELISA) and dot blotting analyses were performed with freezing and thawing treatments during antigen preparation. Dilution of extracts to an appropriate scale significantly reduced non-specific reactions. The resultant PaWB EF-Tu antibody reacted with antigens from plants infected with periwinkle virescence and chinaberry tree witches’-broom phytoplasmas, but not those infected with jujube witches’-broom or bishopwood witches’-broom phytoplasma. The EF-Tu was characteristically localized within the phytoplasmal cytoplasm of infected plant phloem tissues. [ABSTRACT FROM AUTHOR]
- Subjects :
- *PHYTOPLASMA diseases
*PAULOWNIA
*CARRIER protein genetics
Subjects
Details
- Language :
- English
- ISSN :
- 00320862
- Volume :
- 59
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- Plant Pathology
- Publication Type :
- Academic Journal
- Accession number :
- 53323051
- Full Text :
- https://doi.org/10.1111/j.1365-3059.2010.02316.x