COMPARISON BETWEEN DIFFERENTIATION POTENTIAL OF DOG PERIOSTEUM - DERIVED CELLS AND BONE MARROW-DERIVED CELLS TO OSTEOGENIC, CHONDROGENIC AND ADIPOGENIC LINEAGES.

The aim of the present study is to investigate the probability of dog periosteum-derived cells and bone marrow-derived cells to differentiate along the osteogenic, chondrogenic and adipogenic lineages. Results of this experiment aim to highlight the similarities and differences between the cells obtained as a result of periosteum-derived culture and bone marrow-derived cells cultivated in three differentiation media. While the dog bone marrow derived-cells differentiate towards all three lineages, with variable intensity, thus confirming the results of other research studies in this field referring to other species, canine periosteum-derived cells obtained using culture of periosteal explants, differentiate mostly towards the osteoblastic and chondrogenic lineage, and to lesser extent towards the adipogenic lineage; the cells obtained using the digestion method of periosteal fragments positively differentiate towards all the three lineages: osteoblastic, chondrogenic and adipogenic. In addition, it was demonstrated that cryopreserved studied cells have osteogenic, chondrogenic and adipogenic differentiation capacity. [ABSTRACT FROM AUTHOR]