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Regulation of Gadd45γ expression by C/EBP.
- Source :
-
European Journal of Biochemistry . Oct2000 Part 2, Vol. 267 Issue 20, p6180-6187. 8p. 8 Diagrams, 6 Graphs. - Publication Year :
- 2000
-
Abstract
- The Gadd45γ (growth arrest and DNA damage-inducible) gene is activated transcriptionally by at least two kinds of agents: DNA damaging agent such as methyl methanesulfonate (MMS) and UV radiation, or cytokines such as interleukin (IL)-6, IL-2 and granulocyte colony-stimulating factor (G-CSF). To investigate the sequences and transcription factors involved in induction of Gadd45γ after treatment with IL-6, the human gene was cloned and sequenced. We found C/EBP (CCAAT/enhancer-binding protein) family proteins, major transcription factors in the IL-6 signal transduction pathway, could regulate the transcriptional activity of the Gadd45γ promoter. In addition, a noncanonical C/EBP-binding site within the Gadd45γ promoter where C/EBPβ and C/EBPδ could bind, was identified by electrophoretic mobility shift assay (EMSA) and reporter gene analysis. Furthermore, we found a coordinated expression profile between Gadd45γ mRNA and C/EBPs (β and δ) protein during the differentiation of M1 cells: the amount of Gadd45γ transcripts became maximal when both C/EBPβ and C/EBPδ levels were high, on day 1 of differentiation of M1 cells after treatment with IL-6. These findings suggest that mitotic growth arrest coupled to M1 cell differentiation is mediated by C/EBPs stimulation of growth arrest-associated genes such as Gadd45γ. [ABSTRACT FROM AUTHOR]
- Subjects :
- *DNA
*GENE expression
Subjects
Details
- Language :
- English
- ISSN :
- 00142956
- Volume :
- 267
- Issue :
- 20
- Database :
- Academic Search Index
- Journal :
- European Journal of Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 5169482
- Full Text :
- https://doi.org/10.1046/j.1432-1327.2000.01692.x