Lipid Concentration Effects on the Amyloidogenic apoC-II60−70Peptide: A Computational Study.

Molecular dynamics simulations were implemented to investigate the effects of phospholipid concentration on the conformation and dynamics of the amyloidogenic peptide apoC-II60−70. The results showed a progressive reduction in the solvent accessible surface area of apoC-II60−70with increasing lipid concentration, accompanied by increased lipid−peptide interactions. Favorable peptide interaction sites with lipids were found to be the aromatic residues, Tyr63 and Phe67. The high stability of lipid−peptide contacts resulted in reduced conformational flexibility of the peptide. A significant change in the secondary structure of apoC-II60−70peptide was observed with increasing lipid concentration. At lower concentrations (1−3 lipids per peptide), the peptide adopted extended β-strand conformations, caused by contacts with the lipids, which reduced the intramolecular interactions within the peptide. In contrast, a higher lipid concentration (4−6 lipids per peptide) had a restraining effect on the peptide’s flexibility by trapping it in a particular conformation. Such behavior can be suggested as inhibiting fibril formation, because of the lipid-induced inability of the peptide to adopt fibril competent conformations. This finding complements our recent ThT fluorescence results, which revealed that the 4:1 lipid to peptide ratio is sufficient to cause fibril inhibition in apoC-II60−70. [ABSTRACT FROM AUTHOR]