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Tandemly repeated genomic sequence demonstrates inter- and intra-strain genetic variation in Schistosoma japonicum*.
- Source :
-
Tropical Medicine & International Health . May98, Vol. 3 Issue 5. 5 Black and White Photographs. - Publication Year :
- 1998
-
Abstract
- Genetic variability within and among four geographical strains of Schistosoma japonicum was examined using a novel repetitive element. The element, termed Sjrh1.0, was isolated from genomic DNA of a Philippine strain of S. japonicum using a combination of restriction fragment PCR and band-stab PCR. Sjrh1.0 is a tandemly repeated element, the sequence of which appears to be species-specific, in that it hybridized to DNA from S. japonicum but not to DNA from S. mansoni. Its sequence does not match previously deposited sequences in GenBank. When employed as a probe in Southern hybridization analysis, radiolabelled Sjrh1.0 revealed sex-specific and strain-specific differences in genomic DNA of individual worms. We also found individual genetic variation within geographical isolates of the Asian schistosome. [ABSTRACT FROM AUTHOR]
- Subjects :
- *SCHISTOSOMA japonicum
*DNA
*SOUTHERN blot
*BIOCHEMICAL mechanism of action
Subjects
Details
- Language :
- English
- ISSN :
- 13602276
- Volume :
- 3
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- Tropical Medicine & International Health
- Publication Type :
- Academic Journal
- Accession number :
- 5089303
- Full Text :
- https://doi.org/10.1046/j.1365-3156.1998.00228.x