Design of a Protein-Targeting System for Lactic Acid Bacteria.

Designs an expression and export system that enables the targeting of a reporter protein Nuc, a staphylococcal nuclease, to specific locations in Lactococcus lactis. Improvement in protein export; Prevention of interaction of secreted Nuc with the cell wall; Effect of the nature of protein N terminus on protein release in a medium; Applications of protein targeting procedures in various lactic acid bacteria hosts.