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Metarhizium anisopliae host–pathogen interaction: differential immunoproteomics reveals proteins involved in the infection process of arthropods
- Source :
-
Fungal Biology . Apr2010, Vol. 114 Issue 4, p312-319. 8p. - Publication Year :
- 2010
-
Abstract
- Abstract: Metarhizium anisopliae is an entomopathogenic fungus well characterized for the biocontrol of a wide range of plagues. Its pathogenicity depends on the secretion of hydrolytic enzymes that degrade the host cuticle. To identify proteins involved in the infection process and in host specify, immunoproteomic analysis was performed using antiserum produced against crude extract of M. anisopliae cultured in the presence of Rhipicephalus (Boophilus) microplus and Dysdercus peruvianus cuticles. Spots detected using antisera produced against M. anisopliae cultured in cuticles and spore surface proteins, but not with antiserum against M. anisopliae cultured in glucose, were identified so as to give insights about the infection process. An MS/MS allowed the identification of proteases, like elastase, trypsin, chymotrypsin, carboxypeptidase and subtilisin (Pr1A, Pr1I and PR1J), chitinases, DNase I and proline-rich protein. Chymotrypsin and Pr1I were inferred as host specific, being recognized in D. peruvianus infection only. This research represents an important contribution to the understanding the adaptation mechanisms of M. anisopliae to different hosts. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 18786146
- Volume :
- 114
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Fungal Biology
- Publication Type :
- Academic Journal
- Accession number :
- 50227549
- Full Text :
- https://doi.org/10.1016/j.funbio.2010.01.006