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Photodynamic inactivation of Candida albicans by BAM-SiPc.
- Source :
-
Mycoses . May2010, Vol. 53 Issue 3, p215-220. 6p. 1 Black and White Photograph, 1 Diagram, 3 Graphs. - Publication Year :
- 2010
-
Abstract
- Photodynamic therapy is a treatment that combines the use of three non-toxic components, viz. photosensitiser, light and oxygen to cause localised oxidative photodamage. In the present study, the antifungal effect of the photosensitiser, BAM-SiPc, an unsymmetrical bisamino phthalocyanine, was investigated. BAM-SiPc was effective in photo-inactivating Candida albicans in a dose-dependent manner. The cell viability as determined by the clonogenic assay was reduced to c. 10% at 0.02 μmol l−1 BAM-SiPc with a total fluence of 12 J cm−2 at a cell density of 107 cells ml−1. A short incubation time of 5–15 min was sufficient to allow the photosensitiser to exert its optimal antifungal activity. Microscopical analysis showed that BAM-SiPc was effectively internalised by the fungal cells. Photodynamic treatment led to an increase in the intracellular reactive oxygen species level and disturbed the membrane integrity of the fungal cells. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09337407
- Volume :
- 53
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Mycoses
- Publication Type :
- Academic Journal
- Accession number :
- 48998803
- Full Text :
- https://doi.org/10.1111/j.1439-0507.2008.01688.x