Glucose influence on the production of T-2 toxin by Fusarium sporotrichioides

Abstract: Toxigenic isolate of Fusarium sporotrichioides was tested for the T-2 toxin production on PDA plates during 10 days under various glucose concentrations. T-2 toxin was determined by LC–MS and confirmed with LC–MS/MS. This analytical method has been applied, for the first time, to an extensive study of T-2 accumulation. Results showed that the production of this mycotoxin is directly correlated to the concentration of glucose present in the medium. Concentrations of T-2 toxin produced by the strain of F. sporotrichioides ranged from 0 to 1.45mg/kg. The better T-2 production was evidenced in the fermentation operated with 20% of glucose. [Copyright &y& Elsevier]