DEVELOPMENT AND VALIDATION OF UV SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF DIACEREIN IN CAPSULES.

A simple, sensitive, rapid, accurate and precise spectrophotometric method has been developed for the estimation of diacerein in bulk and pharmaceutical dosage forms. Diacerein shows maximum absorbance at 258.5 nm with molar absorptivity of 4.2258×104l/mol/cm. Beer's law was obeyed in the concentration range of 1-10 μg/ml. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.02 μg/ml and 0.07 μg/ml, respectively. The results of recovery studies (98.68-101.11%) indicated that proposed method is accurate and precise for the determination of diacerein in capsules. [ABSTRACT FROM AUTHOR]