Genetic Analysis for the Lack of Expression of the 0157 Antigen in an 0 Rough:H7 Escherichia coli Strains.

Authors :: Rump, Lydia V.
Feng, Peter C. H.
Fischer, Markus
Monday, Steven R.
Source :: Applied & Environmental Microbiology. Feb2010, Vol. 76 Issue 3, p945-947. 3p.
Publication Year :: 2010
Abstract: The O-antigen (rfb) operon and related genes of MA6, an O rough:H7 Shiga-toxigenic Escherichia coli strain, were examined to determine the cause of the lack of 0157 expression. A 1,310-bp insertion, homologous to 1S629, was observed within its gne gene. trans complementation with a functional gne gene from O157:H7 restored O157 antigen expression in MA6. [ABSTRACT FROM AUTHOR]

Subjects :: *ESCHERICHIA coli
*BACTERIAL genetics
*PARASITE antigens
*OPERONS
*NUCLEOTIDE sequence
*GENETIC polymorphisms
*ANTIGEN analysis
*BIOSYNTHESIS
*SEROLOGY
*ALLELES

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