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Direct electrochemistry and electrocatalysis of heme-proteins immobilized in porous carbon nanofiber/room-temperature ionic liquid composite film
- Source :
-
Electrochimica Acta . Mar2010, Vol. 55 Issue 9, p3185-3191. 7p. - Publication Year :
- 2010
-
Abstract
- Abstract: The combination of porous carbon nanofiber (PCNF) and room-temperature ionic liquid (RTIL) provided a suitable microenvironment for heme-proteins to transfer electron directly. Hemoglobin, myoglobin, and cytochrome c incorporated in PCNF/RTIL films exhibited a pair of well-defined, quasi-reversible cyclic voltammetric peaks at about −0.28V vs. SCE in pH 7.0 buffers, respectively, characteristic of the protein heme Fe(III)/Fe(II) redox couples. The cyclic voltammetry and electrochemical impedance spectroscopy were used to characterize the modified electrode. The heme/PCNF/RTIL/CHIT films were also characterized by UV–vis spectroscopy, indicating that heme-proteins in the composite film could retain its native structure. Oxygen, hydrogen peroxide, and nitrite were catalytically reduced at the heme/PCNF/RTIL/CHIT film modified electrodes, showing the potential applicability of the films as the new type of biosensors or bioreactors based on direct electrochemistry of the redox proteins. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 00134686
- Volume :
- 55
- Issue :
- 9
- Database :
- Academic Search Index
- Journal :
- Electrochimica Acta
- Publication Type :
- Academic Journal
- Accession number :
- 48404147
- Full Text :
- https://doi.org/10.1016/j.electacta.2009.12.101