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Simultaneous determination of five-type hepatitis virus antigens in 5min using an integrated automatic electrochemical immunosensor array
- Source :
-
Biosensors & Bioelectronics . Mar2010, Vol. 25 Issue 7, p1658-1662. 5p. - Publication Year :
- 2010
-
Abstract
- Abstract: An integrated automatic electrochemical immunosensor array has been designed for the simultaneous detection of 5-type hepatitis virus antigens (i.e. hepatitis A, hepatitis B, hepatitis C, hepatitis D, and hepatitis E). Initially, 5-type hepatitis virus antibodies were immobilized onto a self-made electrochemical sensor array using nanogold particles and protein A as matrices, and the immunosensor array was then used to capture their corresponding antigens from sample solution with a 1-step capture format. The detection is based on the potential change before and after the antigen-antibody reaction by using a 2-electrode system. The developed immunosensor array with allowed simultaneous determination of 5-type hepatitis virus antigens in 5min. The detection limit of the sensor array was ≤1.0ng/mL for most analytes. The intra- and inter-assay imprecision (CVs) of the sensor array were ≤7.3% and 8.1%, respectively. No nonspecific adsorption was evaluated, and the crossing talk between neighboring sites was ≤7.5% (CV). The developed immunoassay system offers promise for label-free, simple, rapid, cost-effective analysis of multi-analyte. Importantly, the chip-based immunosensor array could open new opportunities for high-throughput multi-analyte immunoassays in point-of-care testing of miniaturized lab-on-a-chip devices. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 09565663
- Volume :
- 25
- Issue :
- 7
- Database :
- Academic Search Index
- Journal :
- Biosensors & Bioelectronics
- Publication Type :
- Academic Journal
- Accession number :
- 48221060
- Full Text :
- https://doi.org/10.1016/j.bios.2009.12.004