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High Yield Expression of Human Recombinant PTH (1-34).
- Source :
-
Current Trends in Biotechnology & Pharmacy . Jan2010, Vol. 4 Issue 1, p568-577. 10p. 4 Black and White Photographs, 1 Chart, 3 Graphs. - Publication Year :
- 2010
-
Abstract
- Human parathyroid hormone (hPTH), synthesized in the parathyroid gland as a linear peptide, is one of the key regulatory molecules in calcium homeostasis and bone resorption. The N terminus region of hPTH (1-34) is a functionally important part of the molecule that is sufficient and necessary for potently executing most of the hormonal actions. Therefore, hPTH (1-34) is considered to be an attractive therapeutic agent in the treatment of osteoporosis. Here, we describe a high yield expression and purification method for the production of hPTH (1-34) from Escherichia coli. The hPTH (1-34) was expressed as a fusion protein in soluble form, and found to be more than 25% of total proteins. The fusion protein was first purified by GST affinity chromatography and, after cleavage of GST with Factor Xa, the peptide (hPTH 1-34) was further purified by reversed phase Source-30™ chromatography. This double purification strategy produced 30mg/l of hPTH (1-34) with purity = 98%. The identity of the purified peptide was confirmed by mass spectrometry and N-terminal sequencing analysis. The biological activity of the peptide was confirmed in the rat osteogenic cell line UMR-106 by measuring cAMP levels, which were identical to hPTH standards, indicating that purified rhPTH (1-34) has full biological activity. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09738916
- Volume :
- 4
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Current Trends in Biotechnology & Pharmacy
- Publication Type :
- Academic Journal
- Accession number :
- 48216823