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An Improved 2-step Liquid Culture System for Efficient In Vitro Shoot Proliferation of Sundew (Drosera rotundifolia L.).
- Source :
-
Scientia Pharmaceutica . Dec2009, Vol. 77 Issue 4, p827-835. 9p. 3 Charts. - Publication Year :
- 2009
-
Abstract
- An efficient procedure for in vitro shoot production of the medicinal plant sundew (Drosera rotundifolia L.) was developed. Of three cytokinins tested, zeatin (ZEA) at a concentration of 2 μM resulted in the formation of large numbers of adventitious shoots on leaf explants. A larger size of the small shoots was achieved by combining a 2-weeks preculture with ZEA (step 1: shoot induction) with a 5-weeks main culture without plant growth regulators (step 2: shoot elongation). Liquid media were superior to semisolid media: An average of 27.4 shoots per leaf explant, and 53.3 shoots per shoot explant were achieved. The 2-step culture system with liquid media permits a comparatively cheap micropropagation of sundew as well as in vitro biomass production, with potential for scale-up. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00368709
- Volume :
- 77
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Scientia Pharmaceutica
- Publication Type :
- Academic Journal
- Accession number :
- 47223901
- Full Text :
- https://doi.org/10.3797/scipharm.0908-03