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Lessons from GNE-deficient embryonic stem cells: sialic acid biosynthesis is involved in proliferation and gene expression.
- Source :
-
Glycobiology . Jan2010, Vol. 20 Issue 1, p107-117. 11p. 1 Diagram, 3 Charts, 8 Graphs. - Publication Year :
- 2010
-
Abstract
- Sialic acids are widely expressed as terminal carbohydrates on glycoconjugates of eukaryotic cells. They are involved in a variety of cellular functions, such as cell adhesion or signal recognition. The key enzyme of sialic acid biosynthesis is the bifunctional UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase (GNE), which catalyzes the first two steps of sialic acid biosynthesis in the cytosol. Inactivation of GNE causes early embryonic lethality. In this study, we analyzed wild-type and GNE-deficient embryonic stem cells from mice. We found for the first time that proliferation is directly correlated with GNE-expression and the cellular sialic acid concentration. Furthermore, we identified growth-related genes that are differentially expressed in GNE-deficient embryonic stem cells compared to wild-type embryonic stem cells. [ABSTRACT FROM PUBLISHER]
Details
- Language :
- English
- ISSN :
- 09596658
- Volume :
- 20
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Glycobiology
- Publication Type :
- Academic Journal
- Accession number :
- 47144799
- Full Text :
- https://doi.org/10.1093/glycob/cwp153