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Study of the transport of thyroid hormone by transporters of the SLC10 family
- Source :
-
Molecular & Cellular Endocrinology . Feb2010, Vol. 315 Issue 1/2, p138-145. 8p. - Publication Year :
- 2010
-
Abstract
- Abstract: Transport of (sulfated) iodothyronines across the plasma membrane is required for their intracellular metabolism. Rat Na+/taurocholate cotransporting polypeptide (Ntcp; Slc10a1) has been identified as an important transporter protein. We demonstrate that among the 7 members of the solute carrier family SLC10, only human SLC10A1 mediates sodium-dependent transport of the iodothyronine T4 and iodothyronine sulfates T3S and T4S. In contrast to SLC10A2-7, cells co-expressing SLC10A1 and the deiodinase D1 demonstrate a dramatic increase in T3S and T4S metabolism. The SLC10A1 substrates taurocholate, DHEAS and E3S inhibit T3S and T4S transport. Furthermore, co-transfection of SLC10A1 with CRYM, a well-known intracellular iodothyronine-binding protein, results in an enhanced intracellular accumulation of T3S and T4S, indicating that CRYM binds iodothyronine sulfates. The present findings indicate that the liver-specific transporter SLC10A1 transports (sulfated) iodothyronines, thereby increasing their intracellular availability. Therefore, SLC10A1 may fulfill a critical step in providing liver D1 with iodothyronine sulfates for rapid degradation. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 03037207
- Volume :
- 315
- Issue :
- 1/2
- Database :
- Academic Search Index
- Journal :
- Molecular & Cellular Endocrinology
- Publication Type :
- Academic Journal
- Accession number :
- 47103380
- Full Text :
- https://doi.org/10.1016/j.mce.2009.08.003