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Anti-inflammatory diterpenes from the seeds of Vitex negundo
- Source :
-
Bioorganic & Medicinal Chemistry . Jan2010, Vol. 18 Issue 1, p175-181. 7p. - Publication Year :
- 2010
-
Abstract
- Abstract: Phytochemical investigation of a dichloromethane-soluble extract of Vitex negundo seeds led to the isolation of five labdane diterpenes, negundoins A–E (1–5), a 9,10-seco-abietane diterpene, negundoin F (6), a sandaracopimara-7,15-diene diterpene, negundoin G (7), and two known diterpene derivatives (8, 9). Their chemical structures were elucidated by detailed spectroscopic analyses on the basis of NMR, IR, and MS data. The anti-inflammatory effects of metabolites 1–7 were also evaluated in vitro. Compounds 3 and 5 were among the most potent inhibitors on nitric oxide production by LPS-stimulated RAW 264.7 macrophages, with IC50 values of 0.12 and 0.23μM, respectively. Further studies revealed that compounds 3 and 5 (5μM) significantly reduced the levels of the iNOS protein to 0.40±0.13% and 41.02±6.02%, respectively, and COX-2 protein to 2.06±0.53% and 26.40±7.43%, respectively. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 09680896
- Volume :
- 18
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Bioorganic & Medicinal Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 47056493
- Full Text :
- https://doi.org/10.1016/j.bmc.2009.11.004