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l-Aspartate as an amino acid neurotransmitter: mechanisms of the depolarization-induced release from cerebrocortical synaptosomes.
- Source :
-
Journal of Neurochemistry . Aug2009, Vol. 110 Issue 3, p924-934. 11p. 6 Graphs. - Publication Year :
- 2009
-
Abstract
- The role ofl-aspartate as a classical neurotransmitter of the CNS has been a matter of great debate. In this study, we have characterized the main mechanisms of its depolarization-induced release from rat purified cerebrocortical synaptosomes in superfusion and compared them with those of the well-known excitatory neurotransmitterl-glutamate. High KCl and 4-aminopyridine were used as depolarizing agents. At 15 mM KCl, the overflows of both transmitters were almost completely dependent on external Ca2+. At 35 and 50 mM KCl, the overflows ofl-aspartate, but not those ofl-glutamate, became sensitive todl-threo-β-benzyloxyaspartic acid (dl-TBOA), an excitatory amino acid transporter inhibitor. In the presence ofdl-TBOA, the 50 mM KCl-evoked release ofl-aspartate was still largely external Ca2+-dependent. Thedl-TBOA insensitive, external Ca2+-independent component of the 50 mM KCl-evoked overflows ofl-aspartate andl-glutamate was significantly decreased by the mitochondrial Na+/Ca2+ exchanger blocker CGP 37157. The Ca2+-dependent, KCl-evoked overflows ofl-aspartate andl-glutamate were diminished by botulinum neurotoxin C, although to a significantly different extent. The 4-aminopyridine-inducedl-aspartate andl-glutamate release was completely external Ca2+-dependent and never affected bydl-TBOA. Superimposable results have been obtained by pre-labeling synaptosomes with [3H]d-aspartate and [3H]l-glutamate. Therefore, our data showing thatl-aspartate is released from nerve terminals by calcium-dependent, exocytotic mechanisms support the neurotransmitter role of this amino acid. [ABSTRACT FROM AUTHOR]
- Subjects :
- *AMINO acids
*NEUROTRANSMITTERS
*SYNAPTOSOMES
*ORGANIC acids
*BOTULINUM toxin
Subjects
Details
- Language :
- English
- ISSN :
- 00223042
- Volume :
- 110
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Journal of Neurochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 45277050
- Full Text :
- https://doi.org/10.1111/j.1471-4159.2009.06187.x