Characterization of the transient species generated in the photoexcitation of benzoic acid, 2-hydroxy-, 2-D-ribofuranosylhydrazide.

Using time-resolved technique of laser flash photolysis (LFP), it has been demonstrated that benzoic acid, 2-hydroxy-, 2-D-ribofuranosylhydrazide (BHR) in aqueous solution can be ionized via a mono-photonic process under 266nm laser, giving a hydrated electron, anion radical that formed by hydrated electron reacts with steady state of BHR, and neutral radical that formed from rapid deprotonation of the radical cation of BHR. The quantum yield of photo-ionization is determined to be 0.024 at room temperature. Furthermore studies on the reaction of BHR with the primary irradiated products of water such as eaq − and ·OH, and with ·N3 have been determined. On the basis of comparison of the results from LFP with that obtained from time-resolved pulse radiolysis, the reaction mechanism has unambiguously identified. [Copyright &y& Elsevier]