Back to Search
Start Over
DNA methylation regulates constitutive expression of Stat6 regulatory genes SOCS-1 and SHP-1 in colon cancer cells.
- Source :
-
Journal of Cancer Research & Clinical Oncology . Dec2009, Vol. 135 Issue 12, p1791-1798. 8p. 1 Black and White Photograph, 1 Chart, 4 Graphs. - Publication Year :
- 2009
-
Abstract
- Stat6 signaling is active in cancer cells and IL-4-induced Stat6 activities or Stat6 activational phenotypes vary among cancer cells. This study aimed at investigating possible mechanism(s) involved in the formation of varying Stat6 activities/phenotypes. Stat6 regulatory genes, SOCS-1 and SHP-1, were examined for mRNA expression using RT-PCR, and their promoter DNA methylation was assayed by methylation-specific PCR in Stat6-phenotyped colon cancer cell lines. DNA methylation was then verified by sequencing. RT-PCR assay and Western blotting were used to detect the expression of SOCS-1 and SHP-1 after demethylation using 5-aza-2′-deoxycytidine. Compared with Stat6null Caco-2 cells, Stat6high HT-29 cells showed decreased constitutive expression of SOCS-1 and SHP-1, which correlated with DNA hypermethylation in these genes’ promoters. Interestingly, demethylation in HT-29 cells recovered the constitutive expression of SOCS-1 and SHP-1. These findings suggest that DNA methylation controls the constitutive expression of negative Stat6 regulatory genes, which may affect Stat6 activities. [ABSTRACT FROM AUTHOR]
- Subjects :
- *COLON cancer
*METHYLATION
*CANCER cells
*MESSENGER RNA
*DNA
*GENETIC regulation
Subjects
Details
- Language :
- English
- ISSN :
- 01715216
- Volume :
- 135
- Issue :
- 12
- Database :
- Academic Search Index
- Journal :
- Journal of Cancer Research & Clinical Oncology
- Publication Type :
- Academic Journal
- Accession number :
- 44311914
- Full Text :
- https://doi.org/10.1007/s00432-009-0627-z