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Positive hyaluronan/PEI/DNA complexes as a target-specific intracellular delivery to malignant breast cancer.
- Source :
-
Drug Delivery . Oct2009, Vol. 16 Issue 7, p357-362. 6p. 2 Diagrams, 1 Chart, 3 Graphs. - Publication Year :
- 2009
-
Abstract
- In the present study, The PEI/DNA (PD) complexes was first prepared with positive surface charge under a suitable N/P ratio of 10. The redundant positive charge was partially and excessively shielded by a polysaccharide, hyaluronic acid (HA), in aqueous solution. The HA/PEI/DNA ternary complexes were characterized by assessing the zeta potential and size, then transferred to MDA-MB-435, MDA-MB-231, and MCF-7 cell lines with different amounts of HA-specific CD44 receptors on the surface. Consequently, The transfection efficiency of all the prepared complexes show a little increased to MCF-7 (low CD44 level) while a large increased to MDA-MB-231 and MDA-MB-435 cells (high CD44 level) with adding HA. Also, when HA:PEI charge ratio was 7.5%, the ternary complexes show the highest transfection efficiency. The prepared ternary complexes exhibited increased 2–13-fold fluorescence intensity and lower cell toxicity compared to the PD (N/P, 10). These results indicated that the positive HA/PEI/DNA ternary complexes (HA:PEI charge ratio, 7.5%) can target malignant breast cancer cells with high CD44 level and might be a promising candidate vector for gene therapy. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10717544
- Volume :
- 16
- Issue :
- 7
- Database :
- Academic Search Index
- Journal :
- Drug Delivery
- Publication Type :
- Academic Journal
- Accession number :
- 44131987
- Full Text :
- https://doi.org/10.1080/10717540903059549