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p16INK4A in routine practice as a marker of cervical epithelial neoplasia
- Source :
-
Gynecologic Oncology . Oct2009, Vol. 115 Issue 1, p127-131. 5p. - Publication Year :
- 2009
-
Abstract
- Abstract: Objective: The aim of this study was to validate the role of p16INK4a immunocytochemistry as a marker of cervical epithelial neoplasia in cytology and biopsy samples. Methods: During the period of 4 years a total of 501 immunocytochemistry stains in cytology and biopsy samples were performed. The cytology smears were obtained from two independent laboratories of women with low-grade squamous intraepithelial lesion interpretation of Pap test or ThinPrep. Additionally, a selection of paraffin blocks from cases diagnosed previously with squamous intraepithelial lesion and cervical cancer were immunostained. Results: Among 312 cytology samples analyzed by immunocytochemistry, 36.2% were positive for p16INK4a. Negative cases were followed-up with conventional Pap smear and 86.9% showed regression. Among positive patients, 14 underwent surgery and 100% exhibited squamous intraepithelial lesion. In additional immunohistochemical analysis of selected biopsies from our archive, we found that the ratio of positivity for p16INK4a increased from 40% in L-SIL/CIN1 to 69.8% in H-SIL/CIN2 to 100% in H-SIL/CIN3 and squamous cell carcinoma. A relatively low number (24%) of cases with squamous metaplasia revealed focal and/or sporadic staining in the superficial layers of the epithelium. All the cases of cervical adenocarcinoma and mesonephric carcinoma were positive for p16INK4a. Conclusion: p16INK4a is a useful additional marker for interpretation of problematic Pap smears and reduces the variability during evaluation of suspicious biopsies of the uterine cervix. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 00908258
- Volume :
- 115
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Gynecologic Oncology
- Publication Type :
- Academic Journal
- Accession number :
- 43976680
- Full Text :
- https://doi.org/10.1016/j.ygyno.2009.06.020