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Epigenetics of multiple myeloma after treatment with cytostatics and gamma radiation
- Source :
-
Leukemia Research . Nov2009, Vol. 33 Issue 11, p1490-1498. 9p. - Publication Year :
- 2009
-
Abstract
- Abstract: Genetic and epigenetic changes in multiple myeloma (MM) correlate with the stage of the disease. Therefore, we investigated how cytostatics and gamma radiation influence MM-associated histone modifications. ChIP-PCR and ChIP-on-chip technologies were used to quantify H3K9 acetylation and H3K9 dimethylation at select loci in MM patients, lymphoblastoid ARH-77, and myeloma MOLP-8 cells. Genome-wide analysis revealed that the cytostatic, melphalan, increased H3K9 acetylation at multiple gene promoters in ARH-77 cells. Melphalan and gamma radiation also influenced histone modification of prognostically important c-myc and CCND1 genes in ARH-77 and MOLP-8 cells. Moreover, H3K9 acetylation at c-myc and CCND1 promoters was increased in individual MM patients after melphalan treatment. Western blotting revealed that these effects were accompanied by changes in c-MYC and cyclin D1 protein levels. Taken together, we showed that cytostatics significantly alter histone modification of tumor-related genes which is indispensable for understanding cancer therapies. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 01452126
- Volume :
- 33
- Issue :
- 11
- Database :
- Academic Search Index
- Journal :
- Leukemia Research
- Publication Type :
- Academic Journal
- Accession number :
- 43872738
- Full Text :
- https://doi.org/10.1016/j.leukres.2009.03.016