Global Gene Analysis of Erigeron canadensis-Treated TNF-α-, IL-4- and IL-1β-Stimulated A549 Human Epithelial Cells.

Background/Aims: This study was conducted to evaluate the anti-inflammatory mechanisms of Erigeron canadensis (EC) on the tumor necrosis factor-α (TNF-α)-, interleukin (IL)-4- and IL-1β-induced stimulation of A549 cells. Methods: In the present study, the anti-inflammatory effects of EC on TNF-α-, IL-4- and IL-1β-induced A549 cells were determined by analyzing eotaxin secretion using ELISA. In addition, the effects of ECon gene expression profiles in stimulated A549 cells were evaluated by microarray analysis. Results: Oligonucleotide microarray analysis revealed that inflammatory-related genes such as NOS1, NOS2A, IL-1β, IL-8 and CSF2 and cell adhesion-related genes such as SELE, MMP3, VCAM1, ICAM1, ITGA7 and ITGB2 were downregulated in EC-treated A549 cells that had been pretreated with TNF-α, IL-4 and IL-1β. In addition, significant decreases in Eotaxin, ICAM, VCAM and IL-8 gene expression were observed in EC-treated A549 cells. Conclusions: EC has an anti-inflammatory effect in stimulated A549 cells. Microarray-based genomic survey is a high-throughput approach that is suitable for the evaluation of gene expression in cell lines that have been treated with EC. Copyright © 2009 S. Karger AG, Basel [ABSTRACT FROM AUTHOR]