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Two-parameter monitoring in a lab-on-valve manifold, applied to intracellular H2O2 measurements.
- Source :
-
Analyst . Jul2009, Vol. 134 Issue 7, p1498-1504. 7p. - Publication Year :
- 2009
-
Abstract
- This work introduces, for the first time, simultaneous monitoring of fluorescence and absorbance using Bead Injection in a Lab-on-valve format. The aim of the paper is to show that when the target species, cells immobilized on a stationary phase, are exposed to reagents under well-controlled reaction conditions, dual monitoring yields valuable information. The applicability of this technique is demonstrated by the development of a Bead Injection method for automated measurement of cell density and intracellular hydrogen peroxide. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00032654
- Volume :
- 134
- Issue :
- 7
- Database :
- Academic Search Index
- Journal :
- Analyst
- Publication Type :
- Academic Journal
- Accession number :
- 43371255