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Tec Kinase Mediating IL-8 Transcription in Monocytes Stimulated with LPS.
- Source :
-
Inflammation . Aug2009, Vol. 32 Issue 4, p265-269. 5p. 5 Graphs. - Publication Year :
- 2009
-
Abstract
- The purpose of present study was to explore the possibility of Tec kinase as a mediator for IL-8 transcription in monocytes stimulated with LPS. Plasmids of mouse Tec kinase IV or Tec kinase IV with inactivating point mutations generated with QuikChange site-directed mutagenesis were co-transfected with IL-8 promoter driven luciferase construct into RAW264.7 cells, then luciferase activity was measured with a luminometer. The results shown Tec kinase could significantly enhance IL-8 transcription. Furthermore, point inactivating mutation in SH2, PH or PTK domain almost completely abolish the effects of Tec kinase on the transcription of IL-8. In the transfection experiment, PD98059, a MEK1 inhibitor, decreased the transcription of IL-8 in a dose dependent pattern. When siRNA for Tec kinase was transfected into THP-1 cells, it could efficiently block the production of IL-8 from THP-1 cells ( p < 0.01) stimulated with LPS. In conclusion, Tec kinase may mediate the transcription of IL-8 in monocyte stimulated with LPS. [ABSTRACT FROM AUTHOR]
- Subjects :
- *MONOCYTES
*TRANSCRIPTION factors
*PLASMIDS
*MUTAGENESIS
*FOCAL adhesion kinase
Subjects
Details
- Language :
- English
- ISSN :
- 03603997
- Volume :
- 32
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Inflammation
- Publication Type :
- Academic Journal
- Accession number :
- 43168709
- Full Text :
- https://doi.org/10.1007/s10753-009-9129-z